Time：2017-12-31 Browse times：92
State Key Laboratory of Natural Medicines,
Key Lab of Drug Metabolism and Pharmacokinetics,
China Pharmaceutical University,
Nanjing, 210009, P. R. of China.
Cell Phone: +86-13815885062
Ph.D. Pharmaceutical Science major (Supervisor: Prof. Lingjun Li),
May 2013School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA
B.S. Chemistry major,
June 2008Sichuan University, Chengdu, Sichuan, P. R. China
Expertise and Research Interests
Biological mass spectrometry, metabolomics, chemical proteomics, drug screening
My research focuses on the developing and implementing an array of novel analytical strategies such as metabolomics and chemical proteomics tools to elucidate the direct targets of natural medicines, and gain new insights into the roles natural products play in the regulation of complex metabolic signaling networks. Emphasis has been placed on constructing a multi-faceted and integrated platform that include herbal chemicalome characterization, new strategy for untargeted and targeted analysis of metabolites, chemical proteomics and new bioinformatics tools to allow large-scale novel metabolite identification, etc. Recently, our group is also developing high throughput screening techniques for new drug targets from natural product pool. While significant effort has been directed to technique and method development, it is the biomedical importance of understanding the metabolic system that ultimately drives us our research to address challenging scientific questions.
Jan. 2016-, State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics,
China Pharmaceutical University, P. R. China
Jul. 2013-Dec. 2015, School of Pharmacy, China Pharmaceutical University, P. R. China
1. University of Wisconsin-Madison Vilas Travel Award (2012)
2. Sichuan University Fellowship (2004-2008)
National Natural Science Foundation of China (grants 81403005); the Natural Science Foundation of Jiangsu province, China (grant BK20140667);the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.
1. Hui Ye #, Jingxin Wang #, Zichuan Tian, James Dowell, Quentin Bremer, Gaoyuan Lu, Brian Baldo, Lingjun Li*. Quantitative Mass Spectrometry Reveals Food Intake-Induced Neuropeptide Level Changes in Rat Brain: Functional Assessment of Selected Neuropeptides as Feeding Regulators. Mol Cell Proteomics. 2017 Nov;16(11):1922-1937 (Cover article).
2. Mengqiu Wu, Hui Ye, Zhiyuan Tang, Chang Shao, Gaoyuan Lu, Baoqiang Chen, Yuyu Yang, Guangji Wang*, Haiping Hao*. p53 dynamics orchestrates with binding affinity to target genes for cell fate decision. Cell Death Dis. 2017 Oct 19;8(10):e3130. IF 6.0
3. Lin Wang #，Hui Ye #，Di Sun，Tuo Meng，Lijuan Cao，Mengqiu Wu，Min Zhao，Yun Wang，Baoqiang Chen，Xiaowei Xu，Guangji Wang *，Haiping Hao**. A metabolic pathway extension approach for metabolomic biomarker identification.，Anal Chem，2017, 89(2):1229-1237 IF 6.3
4. Hua Sang，Gaoyuan Lu，Yazhong Liu，Qiyue Hu，Weifeng Xing，Dongbing Cui，Fang Zhou，Jingwei Zhang，Haiping Hao，Guangji Wang*，Hui Ye**. Conjugation site analysis of antibody-drug-conjugates (ADCs) by signature ion fingerprinting and normalized area quantitation approach using nano-liquid chromatography coupled to high resolution mass spectrometry.，Anal Chim Acta，2017.2.22，22（955）：67~78 IF 5.0
5. Mengqiu Wu #, Hui Ye #, Chang Shao, Xiao Zheng, Qingran Li, Lin Wang, Min Zhao, Gaoyuan Lu, Baoqiang Chen, Jun Zhang, Yun Wang, Guangji Wang*, Haiping Hao**. Metabolomics-proteomics combined approach identifies differential metabolism-associated molecular events between senescence and apoptosis. J Proteome Res. 2017 May 10. IF 4.3
6. Ye H, Wang L, Zhu L, Sun D, Luo X, Wang H, Wang G, Hao H. Stepped collisional energy MSAll: an analytical approach for optimal MS/MS acquisition of complex mixture with diverse physicochemical properties. J Mass Spectrom., 2016, 51(5):328-41. IF 2.5
7. Ye H, Zhu L, Wang L, Liu H, Zhang J, Wu M, Wang G*, Hao H*. Stepped MSAll Relied Transition (SMART): an approach to rapidly determine optimal multiple reaction monitoring mass spectrometry parameters for small molecules. Anal Chim Acta., 51(5):328-41. IF 5.0
8. Ye H, Wang J, Zhang Z, Jia C, Schmerberg C, Catherman AD, Thomas PM, Kelleher NL, Li L*. Defining the Neuropeptidome of the Spiny Lobster Panulirus interruptus Brain Using a Multidimensional Mass Spectrometry-Based Platform. J Proteome Res., 14(11):4776-91. IF 4.2
9. Ye H, Mandal R, Catherman A, Thomas PM, Kelleher NL, Ikonomidou C, Li L*. Top-down proteomics with mass spectrometry imaging: a pilot study towards discovery of biomarkers for neurodevelopmental disorders. PLoS One, 9(4):e92831. IF 3.5
10. Ye H, Wang J, Greer T, Strupat K, Li L.* Visualizing neurotransmitters and metabolites in the central nervous system by high resolution and high accuracy mass spectrometric imaging. ACS Chem. Neurosci., 4(7):1049-56. IF 4.2
11. Ye H, Gemperline E, Venkateshwaran M, Chen R, Delaux PM, Howes-Podoll M, Ané JM, Li L.* MALDI mass spectrometry-assisted molecular imaging of metabolites during nitrogen fixation in the Medicago truncatula-Sinorhizobium meliloti symbiosis. Plant J., 75(1):130-45. IF 6.8
12. Ye H‡, Hui L‡, Kellersberger K, Li L.* Mapping of neuropeptides in the crustacean stomatogastric nervous system by imaging mass spectrometry. J. Am. Soc. Mass Spectrom., 24(1):134-47. IF 3.2
13. Ye H‡, Gemperline E‡, Li L.* A vision for better health: mass spectrometry imaging for clinical diagnostics. Clin. Chim. Acta, 420:11-22. IF 2.8
14. Ye H‡, Greer T‡, Li L.* Probing neuropeptide signaling at the organ and cellular domains via imaging mass spectrometry. J. Proteomics, 75(16):5014-26. IF 4.1
15. Ye H‡, Greer T‡, Li L.* From pixel to voxel: a deeper view of biological tissue by 3D mass spectral imaging. Bioanalysis, 3(3):313-32. IF 3.2
16. Wang J, Ye H, Zhang Z, Xiang F, Girdaukas G, Li L. Advancing matrix-assisted laser desorption/ionization-mass spectrometric imaging for capillary electrophoresis analysis of peptides. Anal Chem., 83(9):3462-9. IF 5.8
17. Xiang F, Ye H, Chen R, Fu Q, Li L. N,N-dimethyl leucines as novel isobaric tandem mass tags for quantitative proteomics and peptidomics. Anal Chem., 82(7):2817-25. IF 5.8
‡ The authors contributed equally to this work.